A Modification of the ABTS• Decolorization Method and an Insight into Its Mechanism

A modification of the ABTS• decolorization assay for plate readers is presented. In our modification, 200 µL ABTS solution absorbance 1.0 at 734 nm was added with an antioxidant and decreased resulted. For comparison activities in kinetic decrease, concentration dependence decrease area under curve are recommended. “Fast” “slow” antioxidants were distinguished: while reactions “fast” completed within seconds, not finished after 6 min. We recommend reaction time 60 min assays such antioxidants, blood plasma plant extracts. Sub-additive interactions between some (ascorbate Trolox, hispidulin glutathione ascorbate) found decolorization; possible reasons discussed.